Journal: Disease Models & Mechanisms
Article Title: Priming of innate antimycobacterial immunity by heat-killed Listeria monocytogenes induces sterilizing response in the adult zebrafish tuberculosis model
doi: 10.1242/dmm.031658
Figure Lengend Snippet: Priming with HKLm significantly reduces mycobacterial loads in adult zebrafish via innate responses. (A) Priming of adult zebrafish with 0.5×10 7 cfu of HKLm 1 day prior to M. marinum infection (27±2 cfu) led to a significant decrease in mycobacterial loads compared to control injection of sterile 1× PBS. The graph shows one representative experiment. Samples were collected at 4 wpi (PBS: n =19, HKLm: n =19). (B) Priming with HKLm 1 day prior to M. marinum infection leads to sterilization of M. marinum in 25% of the wild-type (WT) zebrafish. Clearance percentage in the WT PBS control group was 3.7%. The data were collected from four independent experiments. M. marinum infection doses in the independent experiments were 27±2 cfu, 26±13 cfu, 75±13 cfu and 26±8 cfu. PBS: n =54, HKLm: n =56. (C) Priming of adult zebrafish with HKLm leads to a significant decrease in mycobacterial loads compared to PBS controls already at 2 wpi. Infection dose: 48±8 cfu. PBS: n =11, HKLm: n =12. (D) HKLm priming 1 day prior to M. marinum infection significantly reduced mycobacterial loads in rag1 −/− mutant fish compared to the PBS control group at 4 wpi, indicating a role for innate immune responses. The graph contains a combined result from two separate experiments. M. marinum infection doses were 48±8 cfu and 27±2 cfu. (E) Priming with HKLm 1 day prior to M. marinum infection leads to sterilization of M. marinum in 17% of the rag1 −/− mutant fish. Clearance percentage in the PBS control group was 0%. Data are pooled from two independent experiments. PBS: n =26, HKLm: n =23. (F,G) HKLm priming did not affect cumulative mortality in WT adult fish (F; PBS: n =152, HKLm: n =170), but caused a trend of reduced mortality in HKLm-injected rag1 −/− mutant fish (PBS: n =53, HKLm: n =55). P -values in A, C and D were calculated with a two-tailed non-parametric Mann–Whitney test with GraphPad Prism. Medians for the individual experiments are shown in the figures. The P -values in B, E, F and G were calculated with Fisher's test using GraphPad (QuickCalcs) online software.
Article Snippet: The P -values in B, E, F and G were calculated with Fisher's test using GraphPad (QuickCalcs) online software.
Techniques: Infection, Injection, Mutagenesis, Two Tailed Test, MANN-WHITNEY, Software